Journal: Frontiers in Cell and Developmental Biology

Article Title: Bergenin as a Novel Urate-Lowering Therapeutic Strategy for Hyperuricemia

doi: 10.3389/fcell.2020.00703

Figure Lengend Snippet: Effects of bergenin on the serum levels of inflammatory cytokines. Serum levels of IL-1β (A) , TNF-α (C) , IL-6 (E) , IL-1Ra (G) , and IL-18 (I) were measured by ELISA. Data are presented as mean ± SEM. * P < 0.05 and ** P < 0.01; n = 10. Correlation analysis between SUA levels and serum levels of the pro-inflammatory cytokines IL-1β (B) , TNF-α (D) , IL-6 (F) , IL-1Ra (H) , and IL-18 (J) . (K) mRNA levels of IL-10, Arg-1, TNF-α, and iNOS in RAW-264.7 cells. Data are presented as mean ± SEM. * P < 0.05 and ** P < 0.01.

Article Snippet: The concentrations of IL-1β, TNFα, IL-6, IL-10, and IL-1Ra were determined using the respective ELISA kits (BOSTER, China for IL-1Ra and NEOBIOSCIENCE, China for the rest) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Trauma Induces Emergency Hematopoiesis through IL-1/MyD88 dependent production of G-CSF 1

doi: 10.4049/jimmunol.1801456

Figure Lengend Snippet: IL-1 Drives G-CSF Production and Progenitor Expansion after Injury. A. WT mice were treated with recombinant IL-1RA 1 hour before polytrauma; plasma G-CSF was measured by CBA. B, C. WT mice were treated with 3 doses of IL-1 RA (16 hours before injury, at the time of injury and 8 hours after injury). Bone marrow was harvested 24 hours after injury and analyzed by flow cytometry. B. Representative FACS plots showing progenitors in mice with and without IL-1RA. C. Frequency of LT-HSC, ST-HSC and MPP populations. *=p<0.05 by Mann-Whitney U Test. Data are pooled from 2–3 independent experiments. Individual values shown, horizontal bar/whisker represent mean+/−SD)

Article Snippet: To test this hypothesis, we treated mice with recombinant IL-1 receptor antagonists (IL-1RA, Anikinra, Sobi pharmaceuticals) 1 hour before polytrauma.

Techniques: Recombinant, Flow Cytometry, MANN-WHITNEY, Whisker Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Trauma Induces Emergency Hematopoiesis through IL-1/MyD88 dependent production of G-CSF 1

doi: 10.4049/jimmunol.1801456

Figure Lengend Snippet: IL-1 Drives G-CSF Expression. A. WT mice were treated with antibodies against IL-1α and IL-1β alone or in combination as indicated, or with isotype control antibody, 1 hour before polytrauma. 6 hours after injury plasma G-CSF was measured. Data represented as mean+/−SD, n=4–5/group, data are pooled from 2 independent experiments. *=p<0.05 by Mann-Whitney U Test. B/C. IL-1 analysis in tissues from mice at 6h after injury vs. naive mice. IL-1α (B) and IL-1β (C) were measured by CBA in cell lysates from BM, peritoneal exudate, and blood, and in liver homogenates. Cytokine levels were normalized to total protein content. Data represented as mean+/−SD, n=4/group, data are pooled from 2 independent experiments. *=p<0.05 by Mann-Whitney U Test.

Article Snippet: To test this hypothesis, we treated mice with recombinant IL-1 receptor antagonists (IL-1RA, Anikinra, Sobi pharmaceuticals) 1 hour before polytrauma.

Techniques: Expressing, MANN-WHITNEY